The trainees got the results below.
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Certificate of completion
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Workshop on Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse (CARD & JAX Techniques)
The Workshop on Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse (CARD & JAX Techniques) was held October 15-19, 2018 at The Jackson Laboratory in Bar Harbor, ME, USA. The workshop was organized by Naomi Nakagata and Toru Takeo of the Center for Animal Resources and Development (CARD) at Kumamoto University and Rob Taft, Jennifer Corrigan and Leanne Miceli of The Jackson Laboratory. Fifteen students participated in this 5 day event.
This unique hands‐on workshop taught participants to use Assisted Reproductive Technologies (ARTs) to efficiently manage mouse strains using both CARD and JAX techniques. Topics covered include cryobiology and strain archival, colony expansion, strain rescue, rederivation, genetic stability and how to overcome breeding problems using ARTs. Hands‐on techniques covered include embryo handling, embryo and sperm cryopreservation and recovery, surgical and non‐surgical embryo transfer, In‐vitro fertilization, flushing embryos, vasectomy, superovulation and inducing pseudopregnancy. Aseptic surgical techniques, biomethods and project planning were also be covered. Participants were be taught by leading experts in the field and obtained the information they need to implement ARTs when they returned to their institutions.
CARD & JAX techniques covered are as follows:
One of the hallmarks of this workshop is the opportunity for participants to closely interact with the faculty at the Highseas Conference Center. Highseas is a Georgian style mansion built in 1912 and is perched on the cliffs overlooking the entrance to Frenchman Bay and surrounded by beautiful Acadia National Park. Highseas served as the residence and dining facility for attendees. Having meals together as well as other social activities encourages network development and fosters collaboration. Therefore, the course was a significant training and professional development opportunity.
Feedback from workshop participants was overwhelmingly positive. When asked, “Overall, how would you rate the quality of this course,” 100% of respondents rated the course “excellent”, the highest possible rating on the on Likert scale, where 1 = poor and 5 = excellent. Additionally, one participant commented, “What a wonderful course! The technology set-up was great! I loved the videos and the on-camera demos. The course was amazing! Thank you so much!”
The course commenced with the distribution of certificates of completion and an informal discussion.
We would like to extend a special thanks to the workshop organizers & instructors and our generous sponsors (KYUDO Co, Cosmo Bio, HHMI and Stoelting).
Jennifer Corrigan (The Jackson Laboratory, USA)
The Workshop on Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse (CARD & JAX Techniques) was held October 15-19, 2018 at The Jackson Laboratory in Bar Harbor, ME, USA. The workshop was organized by Naomi Nakagata and Toru Takeo of the Center for Animal Resources and Development (CARD) at Kumamoto University and Rob Taft, Jennifer Corrigan and Leanne Miceli of The Jackson Laboratory. Fifteen students participated in this 5 day event.
This unique hands‐on workshop taught participants to use Assisted Reproductive Technologies (ARTs) to efficiently manage mouse strains using both CARD and JAX techniques. Topics covered include cryobiology and strain archival, colony expansion, strain rescue, rederivation, genetic stability and how to overcome breeding problems using ARTs. Hands‐on techniques covered include embryo handling, embryo and sperm cryopreservation and recovery, surgical and non‐surgical embryo transfer, In‐vitro fertilization, flushing embryos, vasectomy, superovulation and inducing pseudopregnancy. Aseptic surgical techniques, biomethods and project planning were also be covered. Participants were be taught by leading experts in the field and obtained the information they need to implement ARTs when they returned to their institutions.
CARD & JAX techniques covered are as follows:
- Embryo handling device and pipette preparation: CARD technique
- Sperm freezing: CARD & JAX technique
- IVF: CARD & JAX techniques
- Cold storage of cauda epididymis: CARD technique
- Vitrification of unfertilized oocytes: CARD technique
- Cold storage of embryos: CARD technique
- Surgical embryo transfer: JAX technique
- Non-Surgical embryo transfer: ParaTechs NSET technique
- Freezing of embryos:
- Slow freezing method: JAX technique
- Simple vitrification method: CARD technique
One of the hallmarks of this workshop is the opportunity for participants to closely interact with the faculty at the Highseas Conference Center. Highseas is a Georgian style mansion built in 1912 and is perched on the cliffs overlooking the entrance to Frenchman Bay and surrounded by beautiful Acadia National Park. Highseas served as the residence and dining facility for attendees. Having meals together as well as other social activities encourages network development and fosters collaboration. Therefore, the course was a significant training and professional development opportunity.
Feedback from workshop participants was overwhelmingly positive. When asked, “Overall, how would you rate the quality of this course,” 100% of respondents rated the course “excellent”, the highest possible rating on the on Likert scale, where 1 = poor and 5 = excellent. Additionally, one participant commented, “What a wonderful course! The technology set-up was great! I loved the videos and the on-camera demos. The course was amazing! Thank you so much!”
The course commenced with the distribution of certificates of completion and an informal discussion.
We would like to extend a special thanks to the workshop organizers & instructors and our generous sponsors (KYUDO Co, Cosmo Bio, HHMI and Stoelting).
Jennifer Corrigan (The Jackson Laboratory, USA)
